Background Introduction
Taq Antibody is a monoclonal Antibody against Taq enzyme for hot start PCR technology. It matches most Taq enzymes on the market and can inhibit DNA polymerase activity at low temperature, effectively inhibit the non-specific annealing of primer and the non-specific amplification caused by primer dimers. It is simple and convenient to use since it can be inactivated rapidly above 70 ℃, that is, denature in the initial DNA denaturation of PCR reaction.
Recommended Diagnostic Raw Materials
[Product Information]
[Product Advantages]
★Sound Sealing Effect: Inhibition of Taq enzyme activity at 37 ℃ before PCR reaction can reach over 90%.
★ Easy Operation: Before PCR reaction, only 1:1 is needed to add to Taq DNA Polymerase to realize hot start or to add to Taq DNA Polymerase in advance, which can be stable for six months at - 20 ℃.
★ High Compatibility: Good compatibility with Taq polymerase of different brands.
★ Fine purification process and stable between batches;
[Application Instruction]
On the PCR platform, Taq enzyme antibody concentration is 1.5mg/ml with its validity of 5U/ μL, suitable for most Taq enzymes on the market. Our suggestion is to mix it with a volume ratio of 1:1, place it at room temperature for 10 min. It will be inactivated in only 30s at 95 ℃, Taq enzyme activity will be fully released. It is suitable for various hot start PCR and qPCR reactions based on Taq enzyme.
[Product Performance]
1.37 ℃ Enzyme Activity Sealing PCR to Verify the Sealing Effect:
Verification of enzyme sealing activity shall be conducted with PCR system easy to produce primer dimer. PCR system was pretreated at 37 ℃ for 30 min after amplification. Those with Taq enzyme antibody have no primer dimer, and those without Taq enzyme antibody produce primer dimer. Therefore, it is judged that the sealing effect is qualified.
1.Add Taq DNA Polymerase
2-4. Add Taq enzyme antibody of different batches
2.Compared with the same Taq polymerase, the same gene, different brands of Taq enzyme antibodies, and different copy numbers of template, Henderson Taq enzyme antibody batches are stable and have high sealing efficiency.
[Company Background]
Qingdao Henderson Biotechnology Co., Ltd, established in 2016, serves as a professional supplier of core raw materials for in vitro diagnostic reagents and is committed to providing high-quality raw materials for global in vitro diagnostic reagent production enterprises, scientific research institutions and other fields.
The company provides the following staple products: antigens, antibodies, enzymes, etc., widely used in the detection of drug, respiratory infectious diseases (A, B, COVID-19 antigen, COVID-19 antibody, COVID-19 neutralizing antibody, syncytial virus, etc.), other infectious diseases (hepatitis, AIDS, tuberculosis, etc.), markers for tumor, inflammation, myocardial markers, animal diseases detection (dog, cat diseases) and other fields.
The company, after years of development, has established several specialized laboratories and SPF experimental animal bases, a comprehensive and systematic R&D verification platform and formed a professional technical service team to provide personalized customized services and one-stop solutions.
Qingdao Henderson Biotechnology Co., Ltd is willing to build a renowned product brand for all customers with high-quality products and professional services to keep a foothold in the market with its sound competitiveness.
Limited space only allows the introduction of major products. You are welcomed to contact Qingdao Henderson Biotechnology Co., Ltd and acquire trial samples if you need more information.
Qingdao Henderson Biotechnology Co., Ltd.
Official Website: http://www.qdhenderson.com/
Tel: 0532-84670782
E-mail: info@qdhenderson.com